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| Status |
Public on Dec 31, 2023 |
| Title |
Inducible degradation of the Mediator subunit Med19 reveals a dedicated role in the regulation of developmental genes expression |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the Drosophila wing imaginal disc, by using a inductible degradable version of Med19 degradation with RNAseq, we got access to the early consequences of Med19 depletion on gene expression. We showed that less than a quarter of the genes expressed in the wing disc were deregulated after Med19 loss. Strikingly, these genes exhibit a spatio-temporal expression pattern like transcription factors or Notch responsive genes that reveals Med19 dedicated role in the regulation of developmental gene transcription.
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| Overall design |
RNAseq was done on drosophila wing discs from female ub> Med19AID L3 larvae that were either fed with Auxin analog NAA (AIDUbAux) to induce M19 degradation, or not (AIDUb) as a control. A second control was performed to monitor Auxin effect on drosophila wing discs from female Med19AID L3 larvae that were fed with Auxin (AIDAux).
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| |
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| Contributor(s) |
Guillou E, Boube M, Jullien D |
| Citation(s) |
36445897 |
| |
| Submission date |
Jun 14, 2021 |
| Last update date |
Apr 19, 2024 |
| Contact name |
muriel BOUBE-TREY |
| E-mail(s) |
muriel.boube-trey@inserm.fr
|
| Organization name |
CNRS INSERM
|
| Lab |
RESTORE
|
| Street address |
4bis av Hubert Curien
|
| City |
Toulouse |
| ZIP/Postal code |
31100 |
| Country |
France |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA737422 |
| SRA |
SRP323951 |
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