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| Status |
Public on Jun 01, 2023 |
| Title |
Single Cell RNAseq and VDJ sequence of ACE2 reactive and non-reactive B-cells isolated from COVID-19 patients peripheral blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Flow cytometry sorted B-cells reactive to ACE2 peptides isolated from peripheral blood of COVID-19 patients compared with non-reactive B-cells using pooled hashtag barcoding and 10x genomics 5'DGE kit and VDJ recombination of B-cells
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| Overall design |
B-cells isolated from 7 COVID-19 patient's peripheral blood were seperated into ACE2 reactive and non-reactive population using ACE2 peptides. Each of these patient's B-cells were labelled with Hashtag oligo linked to antibody, which allowed to barcode each patient with unique DNA oligo sequence. Then ACE2 reactive B cells from 7 patients were pooled together and loaded on 10x Genomics Chip G using 5'DGE kit to interogate single cell 5DGE (Digital gene expression) and VDJ sequence in B-cells. Similarly, ACE2 non-reactive cells were pooled together.
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| Contributor(s) |
Yegnasubramanian S, Rosen A, Vaghasia A, Casciola-Rosen L |
| Citation missing |
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| |
| Submission date |
Jun 14, 2021 |
| Last update date |
Jun 03, 2023 |
| Contact name |
Srinivasan Yegnasubramanian |
| Organization name |
Johns Hopkins SOM
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| Street address |
1661 Orleans St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21231 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA737416 |
| SRA |
SRP323950 |
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