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Series GSE17632 Query DataSets for GSE17632
Status Public on Aug 17, 2009
Title BxPC-3 cells: growth in different nutrients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The MondoA transcription factor forms a heterocomplex with its obligate partner Mlx to regulate ~75% of glucose-dependent transcription. By mediating glucose-induced activation of thioredoxin-interacting protein (TXNIP), MondoA directly represses glucose uptake. Given the predominant role of MondoA in controlling glucose-dependent transcription and glucose uptake, we asked whether glutamine regulates MondoA activity. Expression profiles from glucose and glutamine starved BxPC-3 cells (-G-Q) were compared with those from cells grown in glucose only (+G-Q), glutamine only (-G+Q) or glucose plus glutamine (+G+Q). As expected, TXNIP expression was highly induced by glucose. However, the addition of glutamine repressed the glucose-dependent induction of TXNIP. We show that glutamine inhibits MondoA-dependent transcriptional activation of TXNIP by triggering the recruitment of a histone deacetylase-dependent corepressor to the amino terminus of MondoA. Consistent with the repression of TXNIP, glucose uptake is elevated in cells grown in the presence of glucose and glutamine. Finally, alpha-ketoglutarate, a tricarboxylic acid cycle intermediate, also blocks MondoA-dependent activation of TXNIP and stimulates glucose uptake. Together, these results suggest that glutamine-dependent mitochondrial anapleurosis stimulates glucose uptake by restricting TXNIP expression via MondoA:Mlx complexes.
Overall design Four growth conditons; four biological replicates
Contributor(s) Ayer D, Kaadige M
Citation(s) 19706488
Submission date Aug 13, 2009
Last update date Feb 22, 2018
Contact name Mohan Rao Kaadige
Phone 801-585-5169
Fax 801-585-6410
Organization name Huntsman Cancer Institute
Department Oncological Sciences
Lab Don Ayer
Street address 2000 Circle of Hope
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (12)
GSM440124 control vs. glucose alone_rep 1
GSM440125 control vs. glucose alone_rep 2
GSM440126 control vs. glucose alone_rep 3
BioProject PRJNA118625

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17632_RAW.tar 191.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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