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| Status |
Public on Jun 05, 2021 |
| Title |
Single cell RNAseq analysis of wildtype and Igfbpl1-/- retina microglia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
IGFBPL1 deficiency leads to progressive retinal neuron loss and phosphorylated Tau accumulation in the brain. To investigate the role of IGFBL1 in retina microglia homestasis and function. Total immune cells were sorted from wildtype and Igfbpl1-/- mice applied for single cell RNAseq analysis by 10X genomics technology.
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| Overall design |
scRNA-seq was performed for microglia isolated from retinas of 10 KO and 10 sex-matched WT mice (at 8-10 months of age), using FACS. Mice were transcardially perfused with saline, and total ~3,500 cells were sequenced to an average of 115,000 reads/cell.
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| Contributor(s) |
Hu G, Chen J |
| Citation(s) |
37527036 |
| |
| Submission date |
Jun 04, 2021 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Guangan Hu |
| E-mail(s) |
gahu@mit.edu
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| Organization name |
MIT
|
| Department |
Koch Institute
|
| Lab |
Chen
|
| Street address |
500 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA735262 |
| SRA |
SRP322796 |
