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Series GSE175587 Query DataSets for GSE175587
Status Public on May 28, 2021
Title Gene expression in tissues of root tips from Pinus pinaster seedlings under ammonium nutrition
Organism Pinus pinaster
Experiment type Expression profiling by high throughput sequencing
Summary In the present study, the gene expression changes in four different tissues of root tips from maritime pine were analyzed. The roots were under two nutritional conditions (only watered or fertilised with 3 mM of ammonium) and harvested after 24 hours of the treatment.
 
Overall design Pine seedlings were subdivided into two groups. The control group was irrigated with 80 mL of water (C), the second group was supplied with 80 mL of 3 mM NH4Cl. Root samples were collected 24 houres after the irrigation. Seedling’s root tips were cut and tissues of 5 - 6 mm were imbibed in a specimen holder with Tissue‐Tek optimal cutting temperature embedding medium (Sakura Finetek, Alphen aan den Rijn, The Netherlands) and immediately frozen in liquid nitrogen for cryostat sectioning. The 24 samples were sequenced on an Illumina NovaSeq 6000 as paired-end reads of 150 bp length.
 
Contributor(s) Ortigosa F, Lobato-Fernández C, Cañas RA
Citation(s) 34724238
Submission date May 26, 2021
Last update date Dec 14, 2021
Contact name Rafael A Cañas
E-mail(s) rcanasp@gmail.com
Organization name Universidad de Málaga
Department Biología Molecular y Bioquímica
Lab Integrative Molecular Biology Lab
Street address Facultad de Ciencias, Campus de Teatinos
City Malaga
ZIP/Postal code E29071
Country Spain
 
Platforms (1)
GPL30195 Illumina NovaSeq 6000 (Pinus pinaster)
Samples (24)
GSM5342162 Root cap at time 24 control experiment 1 [A1T24_C_RC]
GSM5342163 Root cap at time 24 control experiment 3 [A3T24_C_RC]
GSM5342164 Root cap at time 24 control experiment 4 [A4T24_C_RC]
Relations
BioProject PRJNA732944
SRA SRP321495

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE175587_CPM_counts.txt.gz 5.8 Mb (ftp)(http) TXT
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Processed data are available on Series record
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