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| Status |
Public on May 23, 2013 |
| Title |
Expression data from immortal and senescence-programmed Huh7 clones |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Cellular senescence is a tumor suppressor mechanism, and immortalization facilitates neoplastic transformation. Both mechanisms may be highly relevant to hepatocellular carcinoma (HCC) development and its molecular heterogeneity. Cellular senescence appears to play a major role in liver diseases. Chronic liver diseases are associated with progressive telomere shortening leading senescence that is observed highly in cirrhosis, but also in some HCC. We previously described the generation of immortal and senescence-programmed clones from HCC-derived Huh7 cell line.
We used microarrays to detail the global programme of gene expression profiles of immortal and senescent-programmed clones.
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| Overall design |
Immortal C11I and G11I clones were tested after PD 150. Senescence-programmed clones were tested at proliferating presenescent (C3P, G12P) and senescent (C3S, G12S) stages. Proliferating presenescent cells were tested at PD 20-30 with >85% BRDU positivity, and senescent cells were <5% BRDU positive and >50% SABG positive. The clones were plated in triplicate and subjected to RNA extraction.
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| Web link |
http://www.bilkent.edu.tr/~infobil/
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| |
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| Contributor(s) |
Ozturk M |
| Citation(s) |
23691139 |
| |
| Submission date |
Aug 06, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Mehmet Ozturk |
| E-mail(s) |
ozturk@fen.bilkent.edu.tr
|
| Phone |
+90 312 266 45 38
|
| Fax |
+90 312 266 50 97
|
| Organization name |
Bilkent University
|
| Department |
BilGen Genetics and Biotechnology Research Center
|
| Street address |
Faculty of Science Building
|
| City |
Ankara |
| ZIP/Postal code |
06800 |
| Country |
Turkey |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
| GSM437415 |
G11 immortal clone, biological rep1 |
| GSM437416 |
G11 immortal clone, biological rep2 |
| GSM437417 |
G11 immortal clone, biological rep3 |
| GSM437418 |
G12 senescence clone, biological rep1 |
| GSM437419 |
G12 senescence clone, biological rep2 |
| GSM437420 |
G12 senescence clone, biological rep3 |
| GSM437424 |
C1 immortal clone, biological rep1 |
| GSM437425 |
C1 immortal clone, biological rep2 |
| GSM437426 |
C1 immortal clone, biological rep3 |
| GSM437427 |
C3 senescent clone, biological rep1 |
| GSM437428 |
C3 senescent clone, biological rep2 |
| GSM437429 |
C3 senescent clone, biological rep3 |
|
| Relations |
| BioProject |
PRJNA118543 |
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