 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 25, 2021 |
| Title |
Single-cell transcriptomics of peripheral blood reveals a novel B-cell subset in renal allograft recipients with accommodation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Kidney transplantation is a preeminent treatment for end-stage renal disease . The application of immunosuppressants is needed for kidney recipients to avoid allograft rejection but increase the risk of infection, and balance between rejection and infection is an important in clinical to reach the immune accommodation. Here, we use single-cell RNA sequencing to fully assess the immune status of peripheral mononuclear cells in kidney recipients. and compared the differences between kidney recipients and healthy people to describe the characteristics of the immune accommodation of kidney recipients. We found a novel B cell subset (CD19+IGKC+IGLC3lowTCL1A-CD127+) of renal transplant recipients with accommodation was significantly lower than that of healthy people and verified by flow cytometry. which may have potential regulatory potential. Furthermore, we found that IL32 may increase the expression of CD19+IGKC+IGLC3lowTCL1A-CD127+ Bcells. In summary, we found that the CD19+IGKC+IGLC3lowTCL1A-CD127+B subgroup with immunomodulatory potential is inhibited in kidney recipients with accommodation state, and IL-32 has therapeutic potential for this.
|
| |
|
| Overall design |
Sc-RNA-seq for PBMC from kidney recipients, patient with end stage renal disease and healthy control.
3 sample:
title; source name; tissue; age; gender
KTx; kidney recipient; blood; 32; male
HC; healthy control; blood; 30; male
ESRD; end-stage renal disease; blood; 35; male
|
| |
|
| Contributor(s) |
Ming Y, Zhuang Q, Li H |
| Citation(s) |
34658852 |
| |
| Submission date |
May 24, 2021 |
| Last update date |
Nov 18, 2021 |
| Contact name |
皓 - 李 |
| E-mail(s) |
yizhikelihao@126.com
|
| Phone |
+8618874071904
|
| Organization name |
the third Xiangya hospital
|
| Street address |
Changsha Organ Transplantation Center, Third Xiangya Hospital, Changsha, Hunan，China
|
| City |
Changsha |
| State/province |
China |
| ZIP/Postal code |
410003 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA732381 |
| SRA |
SRP321114 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE175429_Combined_sample_DBEC_MolsPerCell.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| GSE175429_sample_Sample_Tag_Calls.csv.gz |
68.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
