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| Status |
Public on Nov 09, 2021 |
| Title |
High-throughput Microwell-seq 2.0 reveals massively multiplexed chemical perturbation |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Combining in-cell RT and Microwell-seq 1.0, we established Microwell-seq 2.0 for cost-effective HTS with scRNA-seq-based phenotyping. To assess the fidelity of the Microwell-seq 2.0, we performed a species-mixing experiment with cultured human (293T) and mouse (3T3) cells. Moreover, we assessed the platform on tissue cells with more heterogeneous cell types. By harnessing the power of Microwell-seq 2.0, we analyzed massively multiplexed chemical perturbation of human embryonic stem cells (hESCs) at the single-cell resolution.
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| Overall design |
To assess the fidelity of the Microwell-seq 2.0, we performed a species-mixing experiment with cultured human (293T) and mouse (3T3) cells. Moreover, we assessed the platform on tissue cells with more heterogeneous cell types. By harnessing the power of Microwell-seq 2.0, we analyzed massively multiplexed chemical perturbation of human embryonic stem cells (hESCs) at the single-cell resolution.
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| Contributor(s) |
Guo G, Chen H, Liao Y, Sun Z |
| Citation(s) |
34750394 |
| |
| Submission date |
May 24, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Guoji Guo |
| Organization name |
Center for Stem Cell and Regenerative Medicine
|
| Department |
Zhejiang University School of Medicine
|
| Street address |
Yuhangtang street 866
|
| City |
Hanzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (4)
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| GPL30440 |
DNBSEQ-T7 (Homo sapiens; Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA732361 |
| SRA |
SRP321102 |
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