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| Status |
Public on Jul 01, 2021 |
| Title |
High-resolution transciptome mapping and N6-methyladenosine modification mapping of RAW264.7 cell lines treated with TCP, TBP or TIP. |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Purpose:Detect transcriptome-wide mapping of m6A modifications by MeRIP-seq between normal and chemical-treated RAW264.7 cells.
Methods:Total RNA was extracted using TRIzol Reagent followed by chemical fragmentation. The fragmented RNA was incubated with anti-m6A antibody. The desired m6A-enriched RNA eluate was used to construct cDNA library in parallel with input control.
Conclusion: Our study revealed the difference of m6A-methylated RNA between normal and TCP/TBP/TIP-treated cells by RNA-seq and MeRIP-seq technologies.
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| Overall design |
Compare the difference of m6A-methyalated RNA between normal and TBP/TCP/TIP treated samples.
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| Contributor(s) |
Huang L, Zhang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 19, 2021 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Zhang Jun |
| E-mail(s) |
3219030654@stu.cpu.edu.cn
|
| Phone |
15651718659
|
| Organization name |
China Pharmaceutical University
|
| Lab |
zhoulab
|
| Street address |
longmiandadao
|
| City |
nanjing |
| State/province |
jiangsu |
| ZIP/Postal code |
211198 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA731051 |
| SRA |
SRP320476 |
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