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| Status |
Public on Nov 17, 2021 |
| Title |
Pre-neoplastic stromal cells drive BRCA1-mediated breast tumorigenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Women with germline mtuation in BRCA1 have increased risk for developing hereditary breast cancer. The role of the epitheilum-assocaited niche during BRCA1-driven tumor intiation remains unclear. Here, we show that the pre-malignant stromal niche promotes epithelial prolifeation and BRCA1-driven cancer intiation in trans. Using single-cell RNA seq analysis of human pre-neoplastic BRCA1 and control breast tissue, we show that stromal cells provide numerous pro-proliferative paracrine signals inducing epithelial proliferation.
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| Overall design |
Library generation for four 10x genomics v1 chemistry, and twenty-four 10x genomics v2 chemistry was performed following the Chromim Singel Cell 3' Reagents Kits v2 User Guide: CFG00052 Rev B. Libraries were sequenced on the Illumina NovaSeq6000 platform targeting approximately 50,000 reads per cell.
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| |
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| Contributor(s) |
Nee K, Nguyen Q, Kessenbrock K |
| Citation(s) |
36914836 |
| |
| Submission date |
May 17, 2021 |
| Last update date |
Apr 23, 2023 |
| Contact name |
Kevin Nee |
| Organization name |
University of California, Irvine
|
| Department |
Biological Chemistry
|
| Lab |
Kai Kessebrbock
|
| Street address |
839 Health Science Road, Sprague Hall 114
|
| City |
IRVINE |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA730582 |
| SRA |
SRP320176 |
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