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| Status |
Public on Dec 13, 2021 |
| Title |
CircZNF609 regulates microtubule dynamics ad tumorigenicity by sustaining CKAP5 protein levels. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Circular RNAs (circRNAs) are widely expressed in eukaryotes and highly regulated in a myriad of biological processes. While many studies indicate their activity as miRNA and protein sponges, little is known about their ability to directly control mRNA homeostasis. We show that a widely expressed circRNA, circZNF609, directly interacts with several mRNAs and increases their stability and/or translation by favouring the recruitment of the RNA-binding protein ELAVL1. Specifically, the interaction with Ckap5 mRNA, that interestingly overlaps the back-splicing junction, regulates microtubule homeostasis in several cancer cell lines and sustains cell-cycle progression. Finally, we show that circZNF609 downregulation increases the sensitivity to several microtubule-targeting cancer drugs in the regulation of microtubule metabolism and that LNA protectors against the Ckap5 pairing region on circZNF609 phenocopies such activity. These data set an example of how the small effects tuned by circZNF609/Ckap5 mRNA interaction might have potent output in tumour growth and drug response.
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| Overall design |
High-throughput sequencing of total RNA from circ-ZNF609 pull-down experiments performed using two sets of specific biotynilated antisense probes targeting ZNF609 exon 2 and control biotynilated antisense probes targeting LacZ bacterial mRNA.
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| Contributor(s) |
Bozzoni I, Rossi F, Beltran M, Damizia M, Grelloni C, Colantoni A, Setti A, Di Timoteo G, Dattilo D, Centron Broco A, Nicoletti C, Lavia P |
| Citation(s) |
34942120 |
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| Submission date |
May 10, 2021 |
| Last update date |
Jan 20, 2022 |
| Contact name |
Francesca Rossi |
| E-mail(s) |
franc.rossi@uniroma1.it
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| Organization name |
Sapienza University of Rome
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| Department |
Dipartimento di Biologia e Biotecnologie "Charles Darwin"
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| Lab |
Irene Bozzoni's Lab
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| Street address |
Piazzale Aldo Moro, 5
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| City |
Rome |
| State/province |
RM |
| ZIP/Postal code |
00185 |
| Country |
Italy |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA728673 |
| SRA |
SRP319187 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174172_RAW.tar |
40.0 Kb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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