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| Status |
Public on Jun 30, 2023 |
| Title |
RNA sequencing of liver tissues from mice treated with or without ischemia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RIPK1 is a critical regulator of cell death and inflammation implicated in a multitude of human inflammatory and degenerative diseases. However, it remains elusive mechanistically whether and how RIPK1 may be activated under ischemia conditions. Methods: Total RNA from the total livers of mice ( WT, Ripk1D138N, TNFR1/2 KO) was purified using Qiagen RNeasy mini (Qiagen) according to the manufacturer’s instructions. Library preparation and sequenceing analysis were performed by Quintara Biosciences. Conclusions: Our study indicates that ischemia can induce RIPK1 kinase-dependent cell death and inflammation.
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| Overall design |
Total RNA was extracted from liver tissues from the following genotypes: WT, Ripk1D138N, TNFR1/2 KO. Each group has three repeats.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/37384704/
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| |
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| Contributor(s) |
Zhang T, Chu C, Wei W |
| Citation(s) |
37384704 |
| |
| Submission date |
May 05, 2021 |
| Last update date |
Sep 29, 2023 |
| Contact name |
Chen Chu |
| E-mail(s) |
chen_chu@dfci.harvard.edu
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| Organization name |
Dana-Farber Cancer Institute
|
| Street address |
450 Brookline Ave, Smith 914
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA727577 |
| SRA |
SRP318600 |
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