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Series GSE173928 Query DataSets for GSE173928
Status Public on Nov 04, 2021
Title Effects of aneurysmal low and supra-high wall shear stress on endothelial gene expression
Organism Sus scrofa
Experiment type Expression profiling by high throughput sequencing
Summary Wall shear stress (WSS) is proposed to influence intracranial aneurysm growth and rupture. Physiological WSS in cerebral arteries is estimated around 20-30 dynes/cm2. The WSS typically observed in human IAs is close to 2 dynes/cm2 for wide-neck aneurysms with a slow recirculating flow and >70 dynes/cm2 in aneurysms with impinging “jet flow”. In this study, we investigated the effects of aneurysmal low and supra-high WSS on endothelial cells.
 
Overall design Primary porcine aortic endothelial cells (PECs) used in this study were cultured in DMEM medium supplemented with 10% fetal calf serum and 100 g/mL heparin. For flow experiments, cells were seeded on 1% gelatin coated Ibidi chambers. For low shear stress experiments (2 dynes/cm2), cells were seeded at 30.000 cells per channel in Ibidi μ-Slide VI 0.4. For supra-high shear stress experiments (80 dynes/cm2) cells were seeded at 100.000 cells per channel in Ibidi μ-Slide I 0.2 Luer. The different laminar shear stress conditions were applied for 48h using an Ibidi flow system. To allow comparison between low and physiological (30 dynes/cm2) shear stress, and between supra-high and physiological shear stress, experiments at 30 dynes/cm2 were performed either in Ibidi μ-Slides VI 0.4 (condition named as 302 dynes/cm2) or in Ibidi μ-Slides I 0.2 Luer (condition named as 3080 dynes/cm2), respectively.
 
Contributor(s) Morel S, Diagbouga MR, Kwak B
Citation(s) 34721060
Submission date May 05, 2021
Last update date Nov 04, 2021
Contact name Sylvain LEMEILLE
Organization name University of Geneva Medical School
Department Department of Pathology and Immunology
Street address 1 rue Michel-Servet
City Geneva
ZIP/Postal code 1211
Country Switzerland
 
Platforms (1)
GPL22475 Illumina HiSeq 4000 (Sus scrofa)
Samples (12)
GSM5283033 PECs 2dynes/cm2_1
GSM5283034 PECs 2dynes/cm2_2
GSM5283035 PECs 2dynes/cm2_3
Relations
BioProject PRJNA727563
SRA SRP318587

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Supplementary file Size Download File type/resource
GSE173928_Raw_counts.txt.gz 422.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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