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Status |
Public on Nov 04, 2021 |
Title |
Effects of aneurysmal low and supra-high wall shear stress on endothelial gene expression |
Organism |
Sus scrofa |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Wall shear stress (WSS) is proposed to influence intracranial aneurysm growth and rupture. Physiological WSS in cerebral arteries is estimated around 20-30 dynes/cm2. The WSS typically observed in human IAs is close to 2 dynes/cm2 for wide-neck aneurysms with a slow recirculating flow and >70 dynes/cm2 in aneurysms with impinging “jet flow”. In this study, we investigated the effects of aneurysmal low and supra-high WSS on endothelial cells.
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Overall design |
Primary porcine aortic endothelial cells (PECs) used in this study were cultured in DMEM medium supplemented with 10% fetal calf serum and 100 g/mL heparin. For flow experiments, cells were seeded on 1% gelatin coated Ibidi chambers. For low shear stress experiments (2 dynes/cm2), cells were seeded at 30.000 cells per channel in Ibidi μ-Slide VI 0.4. For supra-high shear stress experiments (80 dynes/cm2) cells were seeded at 100.000 cells per channel in Ibidi μ-Slide I 0.2 Luer. The different laminar shear stress conditions were applied for 48h using an Ibidi flow system. To allow comparison between low and physiological (30 dynes/cm2) shear stress, and between supra-high and physiological shear stress, experiments at 30 dynes/cm2 were performed either in Ibidi μ-Slides VI 0.4 (condition named as 302 dynes/cm2) or in Ibidi μ-Slides I 0.2 Luer (condition named as 3080 dynes/cm2), respectively.
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Contributor(s) |
Morel S, Diagbouga MR, Kwak B |
Citation(s) |
34721060 |
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Submission date |
May 05, 2021 |
Last update date |
Nov 04, 2021 |
Contact name |
Sylvain LEMEILLE |
Organization name |
University of Geneva Medical School
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Department |
Department of Pathology and Immunology
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Street address |
1 rue Michel-Servet
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City |
Geneva |
ZIP/Postal code |
1211 |
Country |
Switzerland |
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Platforms (1) |
GPL22475 |
Illumina HiSeq 4000 (Sus scrofa) |
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Samples (12)
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Relations |
BioProject |
PRJNA727563 |
SRA |
SRP318587 |