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| Status |
Public on Mar 05, 2022 |
| Title |
Transposon sequencing for pathway-specific unipolar polysaccharide biosynthesis screen in Agrobacterium tumefaciens C58 |
| Organism |
Agrobacterium fabrum str. C58 |
| Experiment type |
Other
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| Summary |
Performed en masse sequencing of transposon insertions among A. tumefaciens mutants deficient in unipolar polysaccharide (UPP) production in two different genetic backgrounds, each one specific for one of the two chemical species of UPP (UPPGlcN and UPPGalN)
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| Overall design |
Screening strains overproduce UPP polysaccharide due to elevated cyclic diguanylate monophosphate (cdGMP, because of deletion of pruA ATU_RS05580 regulator) and are also genetically disabled for production of multiple polysacccharides: cellulose, succinoglycan, Beta 1,2-glucans and Beta 1,3-glucans). Mutants were isolated in this back ground with either the uppY (ATU_RS02370) or the uppW (ATU_RS11495) polysaccharide polymerase genes also deleted.
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| Contributor(s) |
Fuqua C, Onyeziri MC |
| Citation missing |
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| |
| Submission date |
May 04, 2021 |
| Last update date |
Mar 05, 2022 |
| Contact name |
Clay Fuqua |
| E-mail(s) |
cfuqua@indiana.edu
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| Organization name |
Indiana University
|
| Department |
Biology
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| Lab |
425E
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| Street address |
1001 E. Third Street
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| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL30075 |
Illumina NextSeq 500 (Agrobacterium fabrum str. C58) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA727231 |
| SRA |
SRP318411 |
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