|
| Status |
Public on May 05, 2021 |
| Title |
Transcriptome sequencing of human neutrophil |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TcpC is a multifunctional virulence factor of uropathogenic E. coli (UPEC). Neutrophil extracellular trap formation (NETosis) is a crucial anti-infection mechanism of neutrophils. Here we show the influence of TcpC on NETosis and related mechanisms. In situ NETosis of kidneys from pyelonephritis mouse model induced by TcpC-secreting wild-type CFT073 (CFT073wt) and LPS-induced in vitro NETosis in CFT073wt- or recombinant TcpC (rTcpC)-treated neutrophils are inhibited. rTcpC enters neutrophils through caveolin-mediated endocytosis and inhibits LPS-induced production of ROS, proinflammatory cytokines and protein but not mRNA levels of peptidylarginine deiminase 4 (PAD4). rTcpC treatment enhances PAD4 ubiquitination and accumulation in proteasomes. Moreover, in vitro ubiquitination kit analyses suggest that TcpC is a PAD4-targetd E3 ubiquitin-ligase. These data suggest that TcpC inhibits NETosis primarily by serving as an E3 ligase that promotes degradation of PAD4. Our findings provide a novel mechanism underlying TcpC-mediated innate immune evasion.
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| |
|
| Overall design |
TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4
|
| |
|
| Contributor(s) |
Ou Q, Fang J, Pan J |
| Citation(s) |
34108482 |
| |
| Submission date |
May 04, 2021 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Qian Ou |
| E-mail(s) |
21818627@zju.edu.cn
|
| Phone |
15200853947
|
| Organization name |
Zhejiang University
|
| Department |
Medical College
|
| Street address |
Medical College of Zijingang Campus of Zhejiang University
|
| City |
HangZhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310015 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA727104 |
| SRA |
SRP318390 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE173807_0_gene_annotation.txt.gz |
2.1 Mb |
(ftp)(http) |
TXT |
| GSE173807_1_gene_expression_statistics.txt.gz |
393 b |
(ftp)(http) |
TXT |
| GSE173807_1_summary_of_gene.txt.gz |
159 b |
(ftp)(http) |
TXT |
| GSE173807_1_transcript_expression_statistics.txt.gz |
404 b |
(ftp)(http) |
TXT |
| GSE173807_2_gene_expression_interval.txt.gz |
527 b |
(ftp)(http) |
TXT |
| GSE173807_2_mapped_stat.txt.gz |
1.0 Kb |
(ftp)(http) |
TXT |
| GSE173807_2_transcript_expression_interval.txt.gz |
557 b |
(ftp)(http) |
TXT |
| GSE173807_3_mapped_region_stat.txt.gz |
221 b |
(ftp)(http) |
TXT |
| GSE173807_3_transcript_expression_coverage.txt.gz |
392 b |
(ftp)(http) |
TXT |
| GSE173807_4_genes_fpkm_expression.txt.gz |
9.3 Mb |
(ftp)(http) |
TXT |
| GSE173807_5_isoforms_fpkm_expression.txt.gz |
19.0 Mb |
(ftp)(http) |
TXT |
| GSE173807_differentially_expressed_genes.txt.gz |
160 b |
(ftp)(http) |
TXT |
| GSE173807_gene_count_matrix.txt.gz |
780.7 Kb |
(ftp)(http) |
TXT |
| GSE173807_merged.fa.gz |
69.4 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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