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| Status |
Public on Sep 12, 2022 |
| Title |
Crosstalk between macrophages and fibro-adipogenic progenitors during muscle regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The purpose of this study is to investigate how immune cells (macrophages) are closely linked with fibro-adipogenic progenitors (FAPs) during recovery processe.
We report that the depletion of Mrc1+ (gene encoding CD206) M2-like macrophages promoted the recovery of muscle after injury. A total RNA sequence analysis of whole muscle or isolated FAPs revealed that the depletion of Mrc1+ M2-like macrophages resulted in the activation of FAPs. Activated FAPs secrete follistatin (Fst), a promyogenic factor, boosting the recovery process. We further determined that the knockdown of FAPs-derived Fst delayed the recovery process. Mechanistically, Mrc1+ M2-like macrophages-specific TGF-b1 inhibited the activation of FAPs, and the FAPs failed to secrete Fst.
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| Overall design |
mRNA profiles of whole muscle 4,7,14 post-day injury (cardiotoxin [CTX]-induced muscle injury) and mRNA profile of isolated FAPs from 7 post-day injured muscle of wild type (WT) and CD206DTR mice were generated by deep sequencing.
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| Contributor(s) |
Yumiko O, Ichiro M |
| Citation(s) |
36411280 |
| |
| Submission date |
Apr 29, 2021 |
| Last update date |
Dec 12, 2022 |
| Contact name |
Ichiro Manabe |
| E-mail(s) |
manabe-tky@umin.ac.jp
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| Organization name |
Chiba University
|
| Street address |
1-8-1 Inohana, Chuo
|
| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA726154 |
| SRA |
SRP316877 |
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