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Series GSE173337 Query DataSets for GSE173337
Status Public on Aug 23, 2021
Title Cannabinoid receptor 2 is necessary to induce toll-like receptor-mediated microglial activation.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The tight regulation of microglia activity is key for precise responses to potential threats, while uncontrolled and exacerbated microglial activity is neurotoxic. Microglial toll-like receptors (TLRs) are indispensable for sensing different types of assaults and triggering an innate immune response. Cannabinoid receptor 2 (CB2) signaling is a key pathway to control microglial homeostasis and activation, and its activation is connected to changes in microglial activity. We aimed to investigate how CB2 signaling impacts TLR-mediated microglial activation. Here, we demonstrate that deletion of CB2 causes a dampened transcriptional response to prototypic TLR ligands in microglia. Loss of CB2 results in distinct microglial gene expression profiles, morphology, and activation. We show that the CB2-mediated attenuation of TLR-induced microglial activation is p38 MAPK-dependent. Taken together, we demonstrate that CB2 expression and signaling are necessary to fine-tune TLR-induced activation programs in microglia.
Overall design Primary neonatal microglia derived from WT and CB2-/- mice were stimulated for 16h in vitro with LPS/IFN-γ, PolyI:C or CpG to activate TLR4, TLR3 and TLR9, respectively. Subsequently, bulk RNA-sequencing was performed from these samples.
Contributor(s) Reusch N, Ravichandran KA, Olabiyi BF, Komorowska-Müller JA, Hansen JN, Ulas T, Beyer M, Zimmer A, Schmöle A
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Submission date Apr 26, 2021
Last update date Sep 02, 2021
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (2)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (27)
GSM5266035 3012_Unstim_wt
GSM5266036 3013_Unstim_wt
GSM5266037 3014_Unstim_wt
BioProject PRJNA725205
SRA SRP316379

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173337_batch_corrected.txt.gz 5.2 Mb (ftp)(http) TXT
GSE173337_norm_count.txt.gz 4.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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