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Status |
Public on Aug 23, 2021 |
Title |
Cannabinoid receptor 2 is necessary to induce toll-like receptor-mediated microglial activation. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The tight regulation of microglia activity is key for precise responses to potential threats, while uncontrolled and exacerbated microglial activity is neurotoxic. Microglial toll-like receptors (TLRs) are indispensable for sensing different types of assaults and triggering an innate immune response. Cannabinoid receptor 2 (CB2) signaling is a key pathway to control microglial homeostasis and activation, and its activation is connected to changes in microglial activity. We aimed to investigate how CB2 signaling impacts TLR-mediated microglial activation. Here, we demonstrate that deletion of CB2 causes a dampened transcriptional response to prototypic TLR ligands in microglia. Loss of CB2 results in distinct microglial gene expression profiles, morphology, and activation. We show that the CB2-mediated attenuation of TLR-induced microglial activation is p38 MAPK-dependent. Taken together, we demonstrate that CB2 expression and signaling are necessary to fine-tune TLR-induced activation programs in microglia.
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Overall design |
Primary neonatal microglia derived from WT and CB2-/- mice were stimulated for 16h in vitro with LPS/IFN-γ, PolyI:C or CpG to activate TLR4, TLR3 and TLR9, respectively. Subsequently, bulk RNA-sequencing was performed from these samples.
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Contributor(s) |
Reusch N, Ravichandran KA, Olabiyi BF, Komorowska-Müller JA, Hansen JN, Ulas T, Beyer M, Zimmer A, Schmöle A |
Citation missing |
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Submission date |
Apr 26, 2021 |
Last update date |
Sep 02, 2021 |
Contact name |
Joachim Schultze |
E-mail(s) |
j.schultze@uni-bonn.de
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Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
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Department |
Genomics and Immunoregulation
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Street address |
Carl-Troll-Strasse 31
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City |
Bonn |
State/province |
NRW |
ZIP/Postal code |
53115 |
Country |
Germany |
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Platforms (2) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (27)
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Relations |
BioProject |
PRJNA725205 |
SRA |
SRP316379 |