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| Status |
Public on Jul 23, 2024 |
| Title |
Single-cell RNA sequencing reveals a role of Phf2 in promoting the exit of postnatal neural stem cell from shallow quiescence. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Dentate gyrus single cell mRNA profiles of 30-day-old wild-type (WT) and Phf2 conditional knockout (Phf2−/−) mice were generated by single cell sequencing. We performed data filtering: i) for the genes expressed in at least 30 cells and cells with detected expression of at least 200 genes and no more than 8000 genes; ii) cells with the percentage of expressed mitochondrial genes no more than 10%. Potential cell doublets were filtered out using scrublet (https://pypi.org/project/scrublet/). Single cell clustering was done using scanpy package (https://scanpy.readthedocs.io/en/stable/).
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| Overall design |
Single cell mRNA profiles of 30-day old Nestin-Cre Tg wild type (Phf2+/+) control mice and conditional KO (Nestin-Cre Tg, Phf2flox/flox) mutant mice.
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| Contributor(s) |
Grinchuk O, Yoon J, Feng J, Lok K, Shirgaonkar N, Ong D |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 26, 2021 |
| Last update date |
Jul 23, 2024 |
| Contact name |
Oleg Grinchuk |
| E-mail(s) |
oleggwork1@gmail.com
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| Organization name |
IMCB
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| Street address |
61 Biopolis Dr, Proteos
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| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM5265026 |
mouse dentate gyrus cells sample, control |
| GSM5265027 |
mouse dentate gyrus cells sample, cKO |
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| Relations |
| BioProject |
PRJNA725138 |
| SRA |
SRP316335 |
