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| Status |
Public on Apr 26, 2021 |
| Title |
Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator |
| Organisms |
Saccharomyces cerevisiae; synthetic construct |
| Experiment type |
Other
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| Summary |
Activation: A pooled screen for protein fragments that induce transcriptional activation of a reporter GFP gene. A pooled pull-down experiment for protein fragments, expressed with mRNA display, that bind Mediator Med15 or TFIID subcomplex.
This SuperSeries is composed of the SubSeries listed below.
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| Overall design |
Refer to individual Series
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| Citation(s) |
33904398 |
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| Submission date |
Apr 22, 2021 |
| Last update date |
Jul 26, 2021 |
| Contact name |
Adrian L Sanborn |
| E-mail(s) |
a@adriansanborn.com
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| Organization name |
Stanford University
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| Street address |
299 Campus Drive West
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (2) |
| GPL26302 |
NextSeq 550 (Saccharomyces cerevisiae) |
| GPL27609 |
NextSeq 550 (synthetic construct) |
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| Samples (26)
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| GSM5261137 |
Activation screen, Library A+B, Bin 4 |
| GSM5261138 |
Activation screen, Library A+B, Bin 5 |
| GSM5261139 |
Activation screen, Library A+B, Bin 6 |
| GSM5261140 |
Activation screen, Library A+B, Bin 7 |
| GSM5261141 |
Activation screen, Library A+B, Bin 8 |
| GSM5261142 |
Activation screen, Library A+B, INPUT |
| GSM5261143 |
Activation screen, Library D+E, Bin 1 |
| GSM5261144 |
Activation screen, Library D+E, Bin 2 |
| GSM5261145 |
Activation screen, Library D+E, Bin 3 |
| GSM5261146 |
Activation screen, Library D+E, Bin 4 |
| GSM5261147 |
Activation screen, Library D+E, Bin 5 |
| GSM5261148 |
Activation screen, Library D+E, Bin 6 |
| GSM5261149 |
Activation screen, Library D+E, Bin 7 |
| GSM5261150 |
Activation screen, Library D+E, Bin 8 |
| GSM5261151 |
Activation screen, Library D+E, INPUT |
| GSM5261173 |
Pulldown Med15 LibA input |
| GSM5261174 |
Pulldown Med15 LibAB replicate1 |
| GSM5261175 |
Pulldown Med15 LibAB replicate2 |
| GSM5261176 |
Pulldown Med15 LibAB replicate3 |
| GSM5261177 |
Pulldown Med15/TFIID LibB input |
| GSM5261178 |
Pulldown TFIID LibA input |
| GSM5261179 |
Pulldown TFIID LibAB replicate1 |
| GSM5261180 |
Pulldown TFIID LibAB replicate2 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE173149 |
Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator (Activation) |
| GSE173152 |
Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator (Pulldown) |
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| Relations |
| BioProject |
PRJNA723885 |
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