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Series GSE17315 Query DataSets for GSE17315
Status Public on Mar 08, 2012
Title mRNA expression upon reconstitution of miR-130a, miR-203 and miR-205 in prostate cancer cell line LNCaP
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Micro RNAs (miRNAs) miR-130a, miR-203 and miR-205 are jointly downregulated in prostate cancer and act as repressors of AR-signaling. MiRNAs are small non-coding RNAs that regulate the expression of specific mRNA targets mainly by translational repression, mRNA deadenylation or cleavage. Reconstitution of these lost miRNAs in the LNCaP PCa cell line cause morphology changes, growth arrest, and apoptosis, increasing when the miRNAs were co-expressed. Bioinformatic target prediction, mRNA expression and protein expression analysis upon overexpression of these miRNAs congruently identified targets known to be overexpressed in PCa and to be involved in AR trans-activation. This series profiles loss in mRNA expression in LNCaP cells transfected with one of the three miRNAs miR-130a, miR-203 and miR-205 compared to LNCaP cells transfected with a scramble miRNA.
Overall design We analyzed three arrays each for miR-130a, miR-203, miR-205, and a scramble miRNA.
Contributor(s) Boll K, Reiche K, Mörbt N, Kretzschmar AK, Schutt K, Tomm J, Verhaegh G, Schalken J, von Bergen M, Horn F, Hackermüller J
Citation(s) 22391564
Submission date Jul 24, 2009
Last update date Dec 06, 2018
Contact name Kristin Reiche
Organization name Fraunhofer Institute for Cell Therapy and Immunology
Department Diagnostics
Street address Perlickstr. 1
City Leipzig
ZIP/Postal code 04103
Country Germany
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM433191 miR-130a overexpression in LNCaP, biological replicate 1
GSM433192 miR-130a overexpression in LNCaP, biological replicate 2
GSM433193 miR-130a overexpression in LNCaP, biological replicate 3
This SubSeries is part of SuperSeries:
GSE17362 miRNA expression, mRNA expression upon miRNA reconstitution, and direct mRNA target identification in prostate cancer cell lines
BioProject PRJNA123603

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Supplementary file Size Download File type/resource
GSE17315_RAW.tar 18.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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