Expression profiling by high throughput sequencing
Summary
High-content screening identified compounds that promote maturation of human neurons derived from pluripotent stem cells. We sequenced total RNA from neurons treated with these drugs to detemrine their effect at the transcriptome level.
Overall design
Bulk RNA-seq from neurons treated with maturation factors or DMSO for 7 days. 3 Biologial replicates from independent differentiations.