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| Status |
Public on Jul 01, 2021 |
| Title |
Ground tissue circuitry regulates organ complexity in monocot roots [roottissues] |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology like flood tolerance and symbiosis. However, little is known about how cortical layers form outside the highly reduced anatomy of the model Arabidopsis. Here we use single-cell RNAseq to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue-formative SHORT-ROOT (SHR) signaling pathway adjacent to the expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue in monocots that sets up anatomical complexity and a host of key traits.
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| Overall design |
mRNA profiles of Maize root tissues collected using FACS
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| Contributor(s) |
Ortiz-Ramirez C, Coqueiro P, Zhang S, Demesa-Arevalo E, Xu X, Rahni R, Gingeras TR, Jackson D, Gallagher KL, Birnbaum KD |
| Citation(s) |
34855479 |
| BioProject |
PRJNA454730 |
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| Submission date |
Apr 18, 2021 |
| Last update date |
Dec 03, 2021 |
| Contact name |
Kenneth David Birnbaum |
| E-mail(s) |
ken.birnbaum@nyu.edu
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| Phone |
212-998-8257
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| Organization name |
New York University
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| Department |
Biology
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| Lab |
Birnbaum
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| Street address |
12 Waverly Place
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10003 |
| Country |
USA |
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| Platforms (1) |
| GPL30012 |
illumina NextSeq 500 (Zea mays) |
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| Samples (24)
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| GSM5251700 |
Root Tip Protoplast Stained (RPS), Epidermis (E)_1 |
| GSM5251701 |
Root Tip Protoplast Stained (RPS), Epidermis (E)_2 |
| GSM5251702 |
Root Tip Protoplast Stained (RPS), Epidermis (E)_3 |
| GSM5251703 |
Root Tip Protoplast Stained (RPS), Stele (S)_1 |
| GSM5251704 |
Root Tip Protoplast Stained (RPS), Stele (S)_2 |
| GSM5251705 |
Root Tip Protoplast Stained (RPS), Stele (S)_3 |
| GSM5251706 |
Root Tip Protoplast Stained (RPS), Cortex (CO)_1 |
| GSM5251707 |
Root Tip Protoplast Stained (RPS), Cortex (CO)_2 |
| GSM5251708 |
Root Tip Protoplast Stained (RPS), Cortex (CO)_3 |
| GSM5251709 |
Root Tip Protoplast (RP), Endodermis (ED)_1 |
| GSM5251710 |
Root Tip Protoplast (RP), Endodermis (ED)_2 |
| GSM5251711 |
Root Tip Protoplast (RP), Endodermis (ED)_3 |
| GSM5251712 |
Root Tip Protoplast Without Cap (RPW), Control (C)_1 |
| GSM5251713 |
Root Tip Protoplast Without Cap (RPW), Control (C)_2 |
| GSM5251714 |
Root Tip Protoplast Without Cap (RPW), Control (C)_3 |
| GSM5251715 |
Root Tips Without Cap (RTW), Control (C)_1 |
| GSM5251716 |
Root Tips Without Cap (RTW), Control (C)_2 |
| GSM5251717 |
Root Tips Without Cap (RTW), Control (C)_3 |
| GSM5251718 |
Root Tip Protoplast (RP), QC (QC)_1 |
| GSM5251719 |
Root Tip Protoplast (RP), QC (QC)_2 |
| GSM5251720 |
Root Tip Protoplast (RP), QC (QC)_3 |
| GSM5251721 |
Root Tips (RT), Root cap (RC)_1 |
| GSM5251722 |
Root Tips (RT), Root cap (RC)_2 |
| GSM5251723 |
Root Tips (RT), Root cap (RC)_3 |
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| This SubSeries is part of SuperSeries: |
| GSE172302 |
Ground tissue circuitry regulates organ complexity in monocot roots |
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| Relations |
| SRA |
SRP145013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE172280_Maize_roottissues_Normalized.csv.gz |
4.4 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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