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| Status |
Public on Apr 18, 2021 |
| Title |
Transcriptomic analysis for the identification of metabolic pathway genes related to toluene response in Ardisia pusilla |
| Organism |
Ardisia pusilla |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants has been reported, but the mechanism of plant response to toluene is only partially understood. In this study, we performed RNA sequencing (RNA-seq) analysis to detect differential gene expression in toluene-treated and untreated leaves of Ardisia pusilla. A total of 88,444 unigenes were identified by RNA-seq analysis, of which 49,623 were successfully annotated and 4,101 were differentially expressed. Gene ontology analysis revealed several sub-categories of genes related to toluene response, including cell part, cellular process, organelle, and metabolic processes. We mapped the main metabolic pathways of genes related to toluene response and found that the differentially expressed genes were mainly involved in glycolysis/gluconeogenesis, starch and sucrose metabolism, glycerophospholipid metabolism, carotenoid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis. In addition, 53 transcription factors belonging to 13 transcription factor families were identified. We verified 10 differentially expressed genes related to metabolic pathways using quantitative real-time PCR and found that the results of RNA-seq were positively correlated with them, indicating that the transcriptome data were reliable. This study provides insights into the metabolic pathways involved in toluene response in plants.
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| Overall design |
mRNA profiles of toluene-treated and untreated A. pusilla.
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| Contributor(s) |
Lee S, Xu J |
| Citation(s) |
34069484 |
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| Submission date |
Apr 17, 2021 |
| Last update date |
Jul 15, 2021 |
| Contact name |
Su Young Lee |
| E-mail(s) |
lsy8542224@korea.kr
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| Organization name |
Rural Development Administration
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| Department |
Floriculture Research Division, National Institute of Horticultural and Herbal Science
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| Street address |
100, Nongsaengmyeong-ro, Iseo-myeon
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| City |
Wanju-gun |
| State/province |
Jeollabuk-do |
| ZIP/Postal code |
55365 |
| Country |
South Korea |
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| Platforms (1) |
| GPL30010 |
Illumina NovaSeq 6000 (Ardisia pusilla) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA722665 |
| SRA |
SRP315187 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE172268_RAW.tar |
90.3 Mb |
(http)(custom) |
TAR (of XLSX) |
| GSE172268_merge_Annotation_Result.xlsx |
21.3 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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