|
| Status |
Public on Feb 07, 2022 |
| Title |
Determination of sRNAs interacting with vfr mRNA across three Pseudomonas aeruginosa strains |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Determination of the RNA interacting with vfr mRNA at two different time-points during growth on the three strains PAO1, PA14 and IHMA87, representing the three major P. aeruginosa phylogenetic lineages, using rGRIL-seq.
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| Overall design |
rGRIL-seq. Interacting RNAs were ligated in live cell by overexpression of a T4 RNA ligase. After cell lysis, total RNA was isolated and enriched for vfr-containing chimeric transcripts using sDNA probes specific to vfr mRNA.
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| |
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| Contributor(s) |
Trouillon J, Han K, Attrée I, Lory S |
| Citation(s) |
35273147 |
| |
| Submission date |
Apr 12, 2021 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Julian Trouillon |
| Organization name |
ETH Zurich
|
| Department |
Institute of Molecular Systems Biology
|
| Street address |
Otto-Stern-Weg 3
|
| City |
Zurich |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL21297 |
Illumina NextSeq 500 (Pseudomonas aeruginosa) |
|
| Samples (12)
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|
| Relations |
| BioProject |
PRJNA721341 |
| SRA |
SRP314458 |
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