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| Status |
Public on Mar 02, 2022 |
| Title |
Evaluation the targeted nanobubbles mediated LND against hepatocellular carcinoma by sonodynamic therapy with RNA-Seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sonodynamic therapy is an important approach in the treatment of hepatocellular carcinoma. A novel targeted nanobubbles loaded with HMME and LND was structured as sensibilizer, characterized the properties, and studied therapeutic effect on hepatocellular carcinoma. Next-generation high throughput RNA sequencing (RNA-Seq) was performed to accurately analyze the differential expression of mRNAs and lncRNA targets in hepatocellular carcinoma after sonodynamic therapy.
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| Overall design |
HepG2 cells and Huh7 cells were used for the research. The cells were transfected using the nanoparticles with the aid of low-frequency ultrasound (LFUS) exposure. Two groups of cells were analyzed using high-throughput RNA sequencing.
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| Contributor(s) |
Shang H, Chen Y, Wu B, Wang C, Cheng W |
| Citation(s) |
35198599 |
| |
| Submission date |
Apr 11, 2021 |
| Last update date |
Mar 02, 2022 |
| Contact name |
shang haitao |
| E-mail(s) |
haitao_shang@126.com
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| Organization name |
Harbin Medical University Cancer Hospital
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| Department |
ultrasound department
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| Street address |
Haping road
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| City |
Harbin |
| ZIP/Postal code |
150000 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA721203 |
| SRA |
SRP314366 |
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