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| Status |
Public on Jul 14, 2021 |
| Title |
High-throughput sequencing analyze the differentially expressed miRNA of HUVECs after lncRNA uc003pxg.1 knockdown by siRNA compared with NC group |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Sequencing of relevant miRNAs was performed in si-uc003pxg.1-transfected HUVECs and control HUVECs. Fourteen upregulated and 5 downregulated miRNAs were selected based on P-values and fold change values. And cell experiments also showed that miR-25-5p was significantly upregulated in HUVECs compared to normal samples following uc003Pxg.1 knockdown. The luciferase activities of the miR-25-5p mimic + uc003pxg.1 wt group were significantly decreased compared with those of the miR-25-5p mimic + uc003pxg.1 mut group, while there was no significant difference in the luciferase activities between the miR-25-5p mimic and NC samples in the mutant group. This result indicated that uc003pxg.1 interacts with miR-25-5p. This study provides a theoretical basis for the study of the molecular mechanism of uc003pxg.1 in HUVECs.
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| Overall design |
Examination the differentially expressed miRNA of HUVECs after lncRNA uc003pxg.1 knockdown by siRNA and NC group
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| Contributor(s) |
Li P, Sun K |
| Citation(s) |
34212983 |
| |
| Submission date |
Apr 08, 2021 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Ping Li |
| E-mail(s) |
liping121200@126.com
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| Organization name |
The Affiliated Suzhou Hospital of Nanjing Medical University
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| Street address |
242 Guangji Road，Suzhou ，Jiangsu
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| City |
Suzhou |
| ZIP/Postal code |
215008 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA720647 |
| SRA |
SRP314030 |
