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| Status |
Public on Apr 06, 2021 |
| Title |
Circulating miRNAs in serum as biomarkers for early diagnosis of non-small cell lung cancer |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The aim of this study was to explore novel miRNA biomarkers in the serum of lung cancer patients based on miRNA profiling. A miRCURY™ LNA Array was used to identify differentially altered miRNAs in the serum of lung cancer patients (n = 6) and healthy controls (n = 6). Total RNA was isolated using TRI reagent BD (MRCgene, TB-126) according to manufacturer’s instructions. After having passed RNA quantity measurement using the NanoDrop 1000, the samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit and hybridized on the miRCURY™ LNA Array (v.18.0). Following the washing steps the slides were scanned using the Axon GenePix 4000B microarray scanner.Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, significant differentially expressed miRNAs were identified through Volcano Plot filtering. Finally, hierarchical clustering was performed to show distinguishable miRNA expression profiling among samples.
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| Overall design |
miRNA profiling in serum samples were analyzed by the 7th generation of miRCURYTM LNA Array (v.18.0, Exiqon). The 7th generation of miRNA array contains 3100 capture probes, covering all human, mouse and rat microRNAs annotated in miRBase 18.0, as well as all viral microRNAs related to these species. In addition, this array contains capture probes for 25 miRPlus™ human microRNAs. These are proprietary microRNAs not found in miRBase.
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| Contributor(s) |
Zhu X |
| Citation(s) |
34306018 |
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| Submission date |
Apr 05, 2021 |
| Last update date |
Jul 28, 2021 |
| Contact name |
Xiaoxia Zhu |
| E-mail(s) |
zhuxx01@126.com
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| Organization name |
Zhujiang Hospital, Southern Medical University
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| Department |
Radiation Oncology Department
|
| Street address |
No. 253 Gongye Street
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510280 |
| Country |
China |
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| Platforms (1) |
| GPL18058 |
Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA719830 |
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