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| Status |
Public on Dec 15, 2022 |
| Title |
Chronic kidney disease is characterized by expansion of pro-inflammatory, HLA-DR-high intermediate monocytes and by increased monocyte adhesion to endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced and analyzed the transcriptomes of PBMCs from the peripheral blood of 5 kidney transplant patients taken at the time of indicated biopsy. We filtered these data to focus on monocytes and compared these data with previously published monocytes/macrophage transcriptomes from paired biopsies.
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| |
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| Overall design |
10X Chromium and NGS used for scRNA-seq of peripheral blood of transplant patients and previously published scRNA-seq data from paired biopsies to study monocyte and macrophage transcriptomes.
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| |
|
| Contributor(s) |
Malone AF |
| Citation(s) |
36799882 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 DK120953 |
Single Cell Analysis of Kidney Transplant Antibody Mediated Rejection |
WASHINGTON UNIVERSITY |
ANDREW FRANCIS MALONE |
|
| |
| Submission date |
Apr 02, 2021 |
| Last update date |
Aug 22, 2023 |
| Contact name |
andrew malone |
| E-mail(s) |
78.andrew@gmail.com
|
| Phone |
3145045281
|
| Organization name |
Washington University
|
| Street address |
4525 laclede ave
|
| City |
st louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (5)
|
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| Relations |
| BioProject |
PRJNA719352 |
| SRA |
SRP313223 |
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