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| Status |
Public on Jul 17, 2009 |
| Title |
Transcriptional profiling of a novel pro-angiogenic small molecule phthalimide neovascular factor 1 (PNF1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We generated the transcriptional regulatory footprint of phthalimide neovascular factor 1 (PNF1)—a novel synthetic small molecule that exhibits significant in vitro endothelial potency and significant in vivo microvascular network expansion—by performing comparative microarray analysis on PNF1-stimulated (versus control) human microvascular endothelial cells (HMVEC) spanning 1-48 h post-supplementation. We subsequently applied network analysis tools (including substantial libraries of information regarding known associations among network components) to elucidate key signaling components and pathways involved in the PNF1 mechanism-of-action. We identified that PNF1 first induces function of the tumor necrosis factor-alpha (TNF-α) signaling pathway, which in turn affects transforming growth factor-beta (TGF-β) signaling.
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| Overall design |
HMVEC (Cambrex, Walkersville, MD, USA) were cultured in endothelial growth medium 2-microvascular (bulletkit, BioWhittaker, Walkersville, MD, USA) supplemented as directed with 5% fetal bovine serum. The cells (passage 9) were plated at 2.5 x 104 cells/cm2 at 37 degrees Celsius in a humidified chamber with 5% carbon dioxide. They were grown to confluence. After confluence, medium was refreshed, and 30 µM PNF1 or 0.6% dimethyl sulfoxide (DMSO) vehicle control was added to the sample. Total RNA from the PNF1 (n=1 at each timepoint) and control (n=1 at each timepoint) samples was isolated 1, 2, 4, 8, 16, 24 and 48 h post-supplementation using an RNeasy kit (Qiagen, Inc., Valencia, CA, USA) according to the manufacturer's protocol.
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| Contributor(s) |
Wieghaus KA, Gianchandani EP, Paige MA, Brown ML, Papin JA, Botchwey EA |
| Citation(s) |
17723106, 18718940, 19326468 |
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| Submission date |
Jul 15, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Erwin P Gianchandani |
| E-mail(s) |
erwin@virginia.edu
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| Organization name |
University of Virginia
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| Department |
Office of the Vice President for Research
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| Street address |
Box 400301
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22904 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (14)
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| GSM428146 |
Control cells, 1 h post-supplementation. |
| GSM428147 |
Control cells, 2 h post-supplementation. |
| GSM428148 |
Control cells, 4 h post-supplementation. |
| GSM428149 |
Control cells, 8 h post-supplementation. |
| GSM428150 |
Control cells, 16 h post-supplementation. |
| GSM428151 |
Control cells, 24 h post-supplementation. |
| GSM428152 |
Control cells, 48 h post-supplementation. |
| GSM428153 |
PNF1-treated cells, 1 h post-supplementation. |
| GSM428154 |
PNF1-treated cells, 2 h post-supplementation. |
| GSM428155 |
PNF1-treated cells, 4 h post-supplementation. |
| GSM428156 |
PNF1-treated cells, 8 h post-supplementation. |
| GSM428157 |
PNF1-treated cells, 16 h post-supplementation. |
| GSM428158 |
PNF1-treated cells, 24 h post-supplementation. |
| GSM428159 |
PNF1-treated cells, 48 h post-supplementation. |
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| Relations |
| BioProject |
PRJNA119715 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17119_RAW.tar |
65.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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