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| Status |
Public on Apr 01, 2024 |
| Title |
Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing [scRNA-seq engrafted HSPCs] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA-seq analysis for WT and Fli-1 KO engrafted HSPCs (Lineage negative CD45.2+) was performed to assess changes in transcriptional output in HSPCs following Fli-1 deletion and Notch1 overexpression and to determine distribution of cells among the different hematopoietic populations.
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| |
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| Overall design |
Single cell RNA sequencing of WT and Fli-1 KO Notch1 OE lineage depleted HSPCs after 4 month of in vivo engraftment preceded by 6 days of co-culture with a vascular niche.
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| |
|
| Contributor(s) |
Itkin T, Houghton S, Hadland B, Rafii S |
| Citation missing |
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| |
| Submission date |
Mar 29, 2021 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Tomer Itkin |
| E-mail(s) |
toi2003@med.cornell.edu
|
| Organization name |
Weill Cornell Medicine
|
| Department |
Medicine
|
| Lab |
Rafii
|
| Street address |
1300 York Avenue, A-869
|
| City |
NY |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
| GSM5219079 |
Fli1 KO Notch1 OE Lineage depleted cells2 |
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| This SubSeries is part of SuperSeries: |
| GSE169431 |
Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing |
|
| Relations |
| BioProject |
PRJNA718389 |
| SRA |
SRP312666 |
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