Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
Summary
We report the application of different chromatin profiling techniques applied to cell-type specific nuclei obtained with a nuclei immuno-enrichment protocol described in this manuscript, referred as NEI protocol. By optimizing these methods, we generated genome-wide datasets associated with the nuclear RNA transcriptome, chromatin accessibility, and distribution of histone H3 and two H3 two lysine tri-methylation marks, H3K4me3 and H3K36me3 respectively. This study provides a framework for the application of different aspects of chromatin biology using nuclei derived from specific cell types in Drosophila. Further, it demonstrates the low input requirements necessary for these chromatin studies.
Overall design
Photoreceptor neuron-specific profiling of nuclear transcriptome by RNA-seq, chromatin accessibility by Omni-ATAC and histone modification distribution by ChIP-seq and CUT&Tag
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