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| Status |
Public on Jul 01, 2021 |
| Title |
Muscle Overload scRNA-seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this project was to determine which mononuclear cell populations satellite cells communicate with throughout muscle during synergist ablation functional overload. Mouse plantaris muscles from tamoxifen-treated Pax7-tdT, tdT, and Pax7-DTA mice were overloaded for 4-14 days, and a subset of mononuclear cells were submitted for sc-RNA-seq.
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| Overall design |
Pooled plantaris muscles from multiple mice in each condition were used for scRNA-seq. A single cell suspension was made, and a subset of cells were isolated via fluorescent activated cell sorting to probe for the transfer of reporter transcript from satellite cells.
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| Contributor(s) |
Murach K, Policastro B, Peterson C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 14, 2021 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Kevin Murach |
| E-mail(s) |
kmu236@g.uky.edu
|
| Phone |
7049042809
|
| Organization name |
University of Kentucky
|
| Department |
Physical Therapy
|
| Street address |
900 South Limestone CTW 445
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA714402 |
| SRA |
SRP310561 |
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