GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE168872 Query DataSets for GSE168872
Status Public on Jul 01, 2021
Title Muscle Overload scRNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this project was to determine which mononuclear cell populations satellite cells communicate with throughout muscle during synergist ablation functional overload. Mouse plantaris muscles from tamoxifen-treated Pax7-tdT, tdT, and Pax7-DTA mice were overloaded for 4-14 days, and a subset of mononuclear cells were submitted for sc-RNA-seq.
Overall design Pooled plantaris muscles from multiple mice in each condition were used for scRNA-seq. A single cell suspension was made, and a subset of cells were isolated via fluorescent activated cell sorting to probe for the transfer of reporter transcript from satellite cells.
Contributor(s) Murach K, Policastro B, Peterson C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 14, 2021
Last update date Jul 02, 2021
Contact name Kevin Murach
Phone 7049042809
Organization name University of Kentucky
Department Physical Therapy
Street address 900 South Limestone CTW 445
City Lexington
State/province KY
ZIP/Postal code 40536
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM5171736 KY_Mononuclear
GSM5171737 pax7_dta
GSM5171738 pax7_tdt
BioProject PRJNA714402
SRA SRP310561

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168872_Ai9_tdTomato.fasta.gz 1.3 Kb (ftp)(http) FASTA
GSE168872_Ai9_tdTomato.gtf.gz 277 b (ftp)(http) GTF
GSE168872_RAW.tar 117.8 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap