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| Status |
Public on Mar 24, 2022 |
| Title |
Transcriptional control of retinal ganglion cell death after axonal injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: This study aims to the downstream transcriptional networks controlled by JUN and DDIT which are critical for RGC death
Methods: RNA was isolated from the retinas of wild-type mice and mice deficient in Jun, Ddit3, and both Jun and Ddit3 three days after mechanical optic nerve crush injury (CONC), and was subjected to RNA-sequecing.
Results: This study identified downstream transcriptional changes after injury included both neuronal survival and pro-inflammatory signaling that were attenuated to differing degrees by loss of Ddit3, Jun, and Ddit3/Jun.
Conclusion: These data suggest pro-inflammatory signaling in the retina might be secondary to activation of pro-death pathways in RGCs after acute axonal injury. These results determine the downstream transcriptional networks important for apoptotic signaling which may be important for ordering and staging the pro-degenerative signals after mechanical axonal injury.
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| Overall design |
Retina mRNA profiles of wild-type and mice deficient in Jun (Jun-/-), Ddit3 (Ddit3-/-), and both Jun and Ddit3 (Jun-/- Ddit3-/-) three days after mechanical optic nerve crush injury (CONC)
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| Contributor(s) |
Syc-Mazurek SB, Yang HS, Marola OJ, Howell GR, Libby RT |
| Citation(s) |
35296661, 36170836 |
| Submission date |
Mar 12, 2021 |
| Last update date |
Jan 02, 2024 |
| Contact name |
Gareth Howell |
| E-mail(s) |
gareth.howell@jax.org
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| Organization name |
The Jackson Laboratory
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| Street address |
600 Main St
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| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA713995 |
| SRA |
SRP310391 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168789_Glaucoma_all_gene_counts.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE168789_RAW.tar |
48.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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