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| Status |
Public on Sep 01, 2022 |
| Title |
DNA Replication Stress Generates Distinctive landscapes of DNA copy number alterations and chromosome scale losses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Diploid human cell lines were treated with aphidicolin as a model to see how DNA replication stress affects the genome, by identifying which copy number alterations appear in the first cell cycle. RNAseq was used to see whether there is any link between loci with recurrent CNAs and gene transcription in the genes located there.
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| Overall design |
RNAseq analysis was performed on the BJ and RPE-1 cell lines treated with Aphidicolin or DMSO control. Three replicates were analysed per condition.
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| Contributor(s) |
Shaikh N, Mazzagatti A, Maniati E, McClelland S |
| Citation(s) |
36266663 |
| |
| Submission date |
Mar 10, 2021 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Eleni Maniati |
| E-mail(s) |
e.maniati@qmul.ac.uk
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| Organization name |
Queen Mary University of London
|
| Department |
Barts Cancer Institute
|
| Lab |
Cancer and Inflammation
|
| Street address |
John Vane Science Centre, Charterhouse Square
|
| City |
London |
| ZIP/Postal code |
EC1M 6BQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA713395 |
| SRA |
SRP310178 |
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