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| Status |
Public on Nov 02, 2022 |
| Title |
Stromal inflammation is a targetable driver of hematopoietic aging [Microarray analysis] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We performed integrated analyses of hematopoietic stem and progenitor cells as well as bone marrow stromal cells to better understand the underlying mechanisms of blood aging.
To determine how the molecular state of multipotent progenitor (MPP) cells change with age we performed transcriptomic analyses of myeloid-biased MPP3 and lymphoid-biased MPP4 populations from young and old mice.
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| Overall design |
Microarray analysis of 3-5 biological replicated of the indicated MPP populations were isolated from young (8-10 weeks) and old (24 months) wild-type C57BL/6 mice. MPP3s are identified as Lin-/cKit+/Sca-1+/Flk2-/CD48+/CD150-, and MPP4s are identified as Lin-/cKit+/Sca-1+/Flk2+. Young controls listed here are also available under GSE68529.
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| Contributor(s) |
Verovskaya EV, Mitchell CA, Pietras EM, Passegue E |
| Citation(s) |
36650381 |
| |
| Submission date |
Mar 10, 2021 |
| Last update date |
Feb 02, 2023 |
| Contact name |
Emmanuelle Passegue |
| E-mail(s) |
ep2828@cumc.columbia.edu
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| Organization name |
Columbia University Irving Medical Center
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| Department |
Columbia Stem Cell Initiative
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| Street address |
650 W 168th St
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE169162 |
Stromal niche inflammation mediated by IL-1 signaling is a targetable driver of hematopoietic aging |
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| Relations |
| BioProject |
PRJNA713346 |
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