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| Status |
Public on Jun 12, 2024 |
| Title |
Whole transcriptomes analyses of HGC-27 cell line treated with control DMSO, disulfiram, thiram or CX-6258 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To figure out the molecular function of disulfiram, thiram and CX-6258, RNA sequencing was performed to analyze the geome-wide change of disulfiram, thiram and CX-6258 treated HGC-27 cells compared to those of control DMSO treated cells. Methods: Total mRNA was extracted from HGC-27 cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina Hiseq 4000 platform. As a reasult, we got the transcript data using Hisat2 followed by Stringtie. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusion: The results showed disulfiram, thiram and CX-6258 may target distinct combinations of various pathways including Hippo pathway. The Hippo signaling indeed can be targeted by disulfiram, thiram and CX-6258 even though these compounds also interfere with other biological processes.
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| Overall design |
Total mRNA was extracted from HGC-27 cells treated with control DMSO, disulfiram, thiram or CX-6258. The quality was assessed using Agilent 2100, and the profiles were sequenced on the Illumina NovaSeq 6000.
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| Contributor(s) |
Tang Y, Jiao S, Zhou Z |
| Citation(s) |
38657866 |
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| Submission date |
Mar 10, 2021 |
| Last update date |
Jun 12, 2024 |
| Contact name |
yang tang |
| E-mail(s) |
tangyang2013@sibcb.ac.cn
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| Organization name |
Shanghai Institute of Biochemistry and Cell Biology
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| Street address |
Yueyang Road 320
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| City |
shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA713189 |
| SRA |
SRP310089 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168618_disulfiram_thiram_SHAP_transcript.TPM.xls.gz |
3.1 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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