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| Status |
Public on Nov 02, 2022 |
| Title |
Stromal inflammation is a targetable driver of hematopoietic aging [droplet-based scRNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate global changes in the structure of the bone marrow niche with age, we performed droplet-based scRNAseq (10X Genomics) of unfractionated endosteal and central marrow stromal cells (Ter119-/CD45-). 8,735 cells passed QC, and hematopoietic clusters of cells were excluded based on marker gene expression, leaving 2359 cells distributed across 9 distinct clusters for final presentation.
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| Overall design |
Endosteal and central marrow stromal cells from the bone marrow of young (10 weeks) and old (24 months) wild-type C57BL/6 mice were isolated by flow cytometry and subjected to droplet-based scRNAseq (10X Genomics).
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| Contributor(s) |
Verovskaya EV, Mitchell CA, Calero-Nieto FJ, Wang X, Gottgens B, Passegue E |
| Citation(s) |
36650381 |
| |
| Submission date |
Mar 09, 2021 |
| Last update date |
Feb 02, 2023 |
| Contact name |
Emmanuelle Passegue |
| E-mail(s) |
ep2828@cumc.columbia.edu
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| Organization name |
Columbia University Irving Medical Center
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| Department |
Columbia Stem Cell Initiative
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| Street address |
650 W 168th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE169162 |
Stromal niche inflammation mediated by IL-1 signaling is a targetable driver of hematopoietic aging |
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| Relations |
| BioProject |
PRJNA708338 |
| SRA |
SRP310000 |
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