|
| Status |
Public on Jan 16, 2024 |
| Title |
Comparative Ribo-Seq of Staphylococcus aureus harboring A2058-unmodified ribosomes and m6A2058-modified ribosomes |
| Organism |
Staphylococcus aureus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
A total of 10 cDNA libraries were constructed, representing 3 and 2 independent biological replicates of S. aureus strain KES34 (unmodified ribosomes) and KES30 (m6A2058-modified ribosomes), respectively. Translational efficiency (RPF/mRNA ratios),
total mRNA abundance and ribosome density of each ORFs were calculated.
|
| |
|
| Overall design |
Investigating the translational efficiency and fidelity of unmodified- and m6A2058-modified ribosomes in a methicilin-resistant Staphylococcus aureus (MRSA).
|
| |
|
| Contributor(s) |
Shields KE, Yap MF |
| Citation(s) |
38252661 |
| |
| Submission date |
Mar 04, 2021 |
| Last update date |
Feb 14, 2024 |
| Contact name |
Mee-Ngan F. Yap |
| E-mail(s) |
frances.yap@northwestern.edu
|
| Phone |
312-503-3793
|
| Organization name |
Northwestern University Feinberg School of Medicine
|
| Department |
Microbiology-Immunology
|
| Lab |
Searle 3-430
|
| Street address |
320 E Superior St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17452 |
Illumina HiSeq 2000 (Staphylococcus aureus) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA706704 |
| SRA |
SRP309329 |
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