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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 22, 2021 |
| Title |
TIPE2 is a checkpoint in natural killer cell maturation and anti-tumor immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The maturation process determines NK cell functionality. We verified that tumor necrosis factor-alpha-induced protein-8 like-2 (TIPE2) works as a potent negative regulator in NK cell maturation. We found that TIPE2 expression gradually increased during NK cell ontogenesis correlating to their maturation stages in mice. NK-specific TIPE2 deficiency resulted in increased number of mature NK cells. To further confirm the role of TIPE2 in NK cell maturation, we performed single-cell RNA sequencing. Based on the key gene expression of NK cell effector molecules, transcriptional factors, maturation markers, and proliferation marker, we identified NK cell maturation program as Cluster#1->Cluster#2->Cluster#0 by which immature NK cells should go through to gain optimal effector functions at the cost of proliferation ability. According to this model, NK-specific TIPE2 deficient mice contained more NK cells belonging to the terminally mature Cluster#0 than control mice (up to 10% more), which is consistent with what we observed by flow cytometry. Therefore, these results collectively showed that NK-specific TIPE2 deficiency promotes NK cell maturation.
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| Overall design |
Single-cell RNAseq of 6-week old NK-specific TIPE2 deficient mice and control mice
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| Contributor(s) |
Bi J |
| Citation(s) |
34524845, 36725083 |
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| Submission date |
Mar 04, 2021 |
| Last update date |
Feb 10, 2023 |
| Contact name |
Jiacheng Bi |
| E-mail(s) |
jc.bi@siat.ac.cn
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| Organization name |
Shenzhen Institute of Advanced Technology
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| Street address |
1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, P.R.China
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| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA706692 |
| SRA |
SRP309316 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168256_RAW.tar |
1.7 Gb |
(http)(custom) |
TAR (of RDS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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