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Series GSE168256 Query DataSets for GSE168256
Status Public on Sep 22, 2021
Title TIPE2 is a checkpoint in natural killer cell maturation and anti-tumor immunity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The maturation process determines NK cell functionality. We verified that tumor necrosis factor-alpha-induced protein-8 like-2 (TIPE2) works as a potent negative regulator in NK cell maturation. We found that TIPE2 expression gradually increased during NK cell ontogenesis correlating to their maturation stages in mice. NK-specific TIPE2 deficiency resulted in increased number of mature NK cells. To further confirm the role of TIPE2 in NK cell maturation, we performed single-cell RNA sequencing. Based on the key gene expression of NK cell effector molecules, transcriptional factors, maturation markers, and proliferation marker, we identified NK cell maturation program as Cluster#1->Cluster#2->Cluster#0 by which immature NK cells should go through to gain optimal effector functions at the cost of proliferation ability. According to this model, NK-specific TIPE2 deficient mice contained more NK cells belonging to the terminally mature Cluster#0 than control mice (up to 10% more), which is consistent with what we observed by flow cytometry. Therefore, these results collectively showed that NK-specific TIPE2 deficiency promotes NK cell maturation.
Overall design Single-cell RNAseq of 6-week old NK-specific TIPE2 deficient mice and control mice
Contributor(s) Bi J
Citation(s) 34524845, 36725083
Submission date Mar 04, 2021
Last update date Feb 10, 2023
Contact name Jiacheng Bi
Organization name Shenzhen Institute of Advanced Technology
Street address 1068 Xueyuan Avenue, Shenzhen University Town, Shenzhen, P.R.China
City Shenzhen
ZIP/Postal code 518055
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5135062 Spleen-WT_scRNA-seq
GSM5135063 Spleen-KO_scRNA-seq
BioProject PRJNA706692
SRA SRP309316

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Supplementary file Size Download File type/resource
GSE168256_RAW.tar 1.7 Gb (http)(custom) TAR (of RDS)
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Raw data are available in SRA
Processed data provided as supplementary file

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