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| Status |
Public on Oct 31, 2021 |
| Title |
Single-Cell RNA-Seq reveals cellualr heterogeneity and gene expression difference between ischemic and hemorrhagic stroke brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cluster analysis using nonlinear dimensionality reduction ([tSNE]) revealed the differences in global gene expression profiles of healthy and injured striatum, and identified clusters of cells with unique genetic signatures in both ischemic brain and hemorrhagic brain. Genes with p-value < 0.05 and fold change ≥1.5 were regarded as differentially expressed genes (DEGs). For astrocytes, 135 DEGs were downregulated in hemorrhagic stroke compared to ischemic stroke. The secondary profiling of astrocytic subtypes yielded 10 different subtypes with distinct functional cell identities. For microglia, tSNE map indicated the distribution and proportion of microglia/macrophage were very similar in in the ischemic and hemorrhagic stroke models. We obtained 75 DEGs in total (hemorrhagic stroke vs. ischemic stroke, 54 were upregulated, 21 were downregulated) .
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| Overall design |
comparison of gene exprssion difference between ischemic and hemorrhagic brain in mice
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| Contributor(s) |
Shi X, Tang Y, Yang G |
| Citation(s) |
34836962 |
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| Submission date |
Feb 26, 2021 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Xiaojing Shi |
| E-mail(s) |
shixiaojing2017@sjtu.edu.cn
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| Organization name |
Shanghai Jiao Tong University
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| Street address |
1954 Huashan Rd.
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| City |
Shanghai |
| State/province |
Please Select |
| ZIP/Postal code |
200030 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA705094 |
| SRA |
SRP308347 |
