GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE16727 Query DataSets for GSE16727
Status Public on Feb 01, 2010
Title Human keratinocytes exposed to tungsten carbide and tungsten carbide cobalt nanoparticles and cobalt chloride
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Comparison of transcriptional profiles after exposure of HaCaT cells to WC or WC-Co nanoparticles and respective amount of free cobalt in form of CoCl2 after 3 hours and 3 days of exposure. Genes responding especially to nanoparticles or leached cobalt ions should be determined.
Overall design Common refernce: pool of 80 RNA Samples of HaCaT cells (treated and not treated), Cy5 labeled. Samples: RNA of HaCaT cells treated 3 hours and 3 days with 30 µg/ml WC nanoparticles, or 33 µg/ml WC-Co nanoparticles (3 µg/ml cobalt content), or 51 µM CoCl2 (equals 3 µg/ml), or water as control, 5 biological replicates for each treatment, all samples were labeled with Cy3 and hybridised together with the common reference.
Contributor(s) Busch W
Citation(s) 20105288
Submission date Jun 19, 2009
Last update date Feb 22, 2018
Contact name Wibke Busch
Phone 00493412351515
Organization name UFZ Helmholtz Centre for Environmental Research
Department Bioanalytical Ecotoxicology
Lab Scholz
Street address Permoser Str. 15
City Leipzig
ZIP/Postal code 04318
Country Germany
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (40)
GSM419105 H2O 3h 1
GSM419106 H2O 3h 2
GSM419107 H2O 3h 4
BioProject PRJNA117493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16727_RAW.tar 471.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap