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| Status |
Public on Apr 20, 2022 |
| Title |
Gene Expression data from HuR-RIP-microarray from human PDAC, Mia PaCa-2 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RNA binding protein, Human Antigen R (HuR/ELAVL1), regulates mRNA stability of key species involved in pancreatic ductal adenocarcinoma (PDAC) cell survival under conditions of chemotherapeutic stress, hypoxia, low glucose environment. We used RNA immunoprecipitation-microarray or RNP-IP from cytoplasmic extracts of PDAC cell lines exposed to chemotherpaeutic stress (olaparib and gemcitabine) to evaluate whole transcriptome gene expression profile associated with HuR and find novel trargtes associated with DNA repair functions of HuR.
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| Overall design |
Cells were treated with chemotherapeutic stressors for 16 hours and cytoplamic HuR was immunoprecipitated using RNP-IP protocol and RNA extracted. Bound RNA were microarrayed using human Clariom D whole transcriptome assays.
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| Contributor(s) |
Brody JR, Jain A, McCoy MM, Addya S, Brown SZ, Pelz C, Sears RC, Yeo CJ |
| Citation(s) |
35406624, 37089813 |
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| Submission date |
Feb 17, 2021 |
| Last update date |
May 12, 2023 |
| Contact name |
Aditi Jain |
| Organization name |
Thomas Jefferson University
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| Department |
Surgery
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| Street address |
1015 Walnut Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19380 |
| Country |
USA |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA702450 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE166951_RAW.tar |
275.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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