|
| Status |
Public on May 20, 2021 |
| Title |
Sequencing of miRXplore Universal Reference (Miltenyi Biotec) RNA using mDOT-seq |
| Organism |
synthetic construct |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In present study we optimized and validated the mDOT-seq approach with miRXplore Universal Reference (Miltenyi Biotec) RNA. The results revealed that the 3' adapters with alkyne moieties attached to the fifth carbon atom of the second cytosine or uracil in ACTC and CUCC sequences, respectively, are best suited for the preparation of RNA sequencing libraries.
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| |
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| Overall design |
Using mDOT-seq eight samples with four different 3' alkyne-adapters (two experimental replicates each) were prepared and analysed.
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| Contributor(s) |
Mickutė M, Kvederavičiūtė K, Vilkaitis G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA680672 |
| |
| Submission date |
Feb 17, 2021 |
| Last update date |
May 20, 2021 |
| Contact name |
Kotryna Kvederaviciute |
| E-mail(s) |
kotryna.kvederaviciute@mif.vu.lt
|
| Organization name |
Vilnius University
|
| Department |
Life Sciences Center
|
| Lab |
Institute Of Biotechnology
|
| Street address |
Sauletekio av. 7
|
| City |
Vilnius |
| ZIP/Postal code |
10257 |
| Country |
Lithuania |
| |
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| Platforms (1) |
| GPL27609 |
NextSeq 550 (synthetic construct) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE166932 |
Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs in probiotic Lactobacillus casei |
|
| Relations |
| SRA |
SRP295248 |
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