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| Status |
Public on Feb 11, 2022 |
| Title |
Increased expression of p53 with APR-246 (eprenetapopt) reprograms tumor-associated macrophages to promote the efficacy of immune checkpoint blockade |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Expression of genes in sorted CD4, CD8 and non-T CD45 cells isolated from TME of B16 tumors in wildtype B6, APR-246 treated wildtype B6 and Super p53 mice.
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| Overall design |
mice bearing B16 tumors were implanted in super p53 or WT mice. WT mice were treated with vehicle or APR-246 starting day 7 for 6 days and single cell suspensions of the tumors were obtained and antibody stained for CD45, CD8. CD4 and live/dead. CD45+ CD8+, CD45+CD8+ and CD45+CD4-CD8- sorted and RNA was extracted with the PAXgene RNA kit and purified in accordance with the manufacturer’s protocols. RNA was assessed with the Agilent BioAnalyzer for quantity and quality.
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| Contributor(s) |
Ghosh A, Mergoub T, Ho Y |
| Citation(s) |
36106631 |
| |
| Submission date |
Feb 11, 2021 |
| Last update date |
Oct 04, 2022 |
| Contact name |
Yu-Jui Ho |
| E-mail(s) |
hoy@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Cancer Biology & Genetics Program
|
| Street address |
417 E 68th St
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (37)
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| Relations |
| BioProject |
PRJNA701459 |
| SRA |
SRP305909 |
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