NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE166544 Query DataSets for GSE166544
Status Public on Jul 07, 2021
Title The molecular basis of coupling between poly(A)-tail length and translational efficiency
Organisms Xenopus laevis; Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary In animal oocytes and early embryos, mRNA poly(A)-tail length strongly influences translational efficiency (TE), but later in development this coupling between tail length and TE disappears. Here, we elucidate how this coupling is first established and why it disappears. Overexpressing cytoplasmic poly(A)-binding protein (PABPC) in frog oocytes specifically improved translation of short-tailed mRNAs, thereby diminishing coupling between tail length and TE. Thus, coupling requires limiting PABPC, implying that in coupled systems longer-tail mRNAs better compete for limiting PABPC. In addition to expressing excess PABPC, post-embryonic cells had two other properties that prevented strong coupling: terminal-uridylation-dependent destabilization of mRNAs lacking bound PABPC, and a regulatory regime wherein PABPC contributes minimally to TE. Thus, these results revealed three fundamental mechanistic requirements for coupling and defined the context-dependent functions for PABPC, in which this protein promotes TE but not mRNA stability in coupled systems and mRNA stability but not TE in uncoupled systems.
 
Overall design RNA-seq, Ribo-seq TAIL-seq, PAL-seq
 
Contributor(s) Xiang K, Bartel D
Citation(s) 34213414
Submission date Feb 10, 2021
Last update date Jul 08, 2021
Contact name David Bartel
Organization name Whitehead Institute
Street address 455 Main Street
City Cambridge
ZIP/Postal code 02142
Country USA
 
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18936 Illumina HiSeq 2500 (Xenopus laevis)
Samples (79)
GSM5074337 frog_oocyte_water_RiboZeroGold_RNA_seq
GSM5074338 frog_oocyte_eGFP_RiboZeroGold_RNA_seq
GSM5074339 frog_oocyte_PABPC1_RiboZeroGold_RNA_seq
Relations
BioProject PRJNA701262
SRA SRP305686

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166544_RAW.tar 1.1 Tb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap