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| Status |
Public on Jun 03, 2024 |
| Title |
Small Nucleolar RNAs in the Dlk1-Gtl2 Imprinting Locus Maintains LT-HSC Self-renewal by Regulation of rRNA Modification and Translation [RiboMeth-seq] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Small nucleolar RNA (snoRNA) are non-coding RNAs, which participate in the cleavage and chemical modification of ribosomal RNAs (rRNAs) and small nuclear RNAs. However, the roles of snoRNA in homeostasis of hematopoietic stem cells(HSCs) have not been studied. We isolated four hematopoietic stem and progenitor cells (HSPCs) (LT-HSCs, IT-HSCs, ST-HSCs, and MPPs) from the bone marrow (BM) of C57BL/6 mice and performed small RNA-seq. We found SnoRNAs belonging to SNORD113-114 cluster were specifically enriched in LT-HSCs, and their expression decreased dramatically with HSC differentiation. To explore function of snoRNAs in SNORD113-114 cluster in HSCs, we established maternally KO mice by CRISPR-Cas9 technology. Here, we used RiboMeth-seq to examine rRNA 2’-O-me modification changes influenced by SNORD113-114 cluster KO.
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| Overall design |
Examination of rRNA 2’-O-me modification in Lin- cells from WT/Mat KO mice.
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| Contributor(s) |
Wang H, Zhang Z, Han Y, Jiang P, Qian P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 10, 2021 |
| Last update date |
Jun 03, 2024 |
| Contact name |
Jiang Penglei |
| E-mail(s) |
jpl3982233@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Department |
School of basic medicine science
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| Street address |
Yuhangtang Road 866
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE166518 |
Small Nucleolar RNAs in the Dlk1-Gtl2 Imprinting Locus Maintains LT-HSC Self-renewal by Regulation of rRNA Modification and Translation |
|
| Relations |
| BioProject |
PRJNA701174 |
| SRA |
SRP305626 |
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