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| Status |
Public on Mar 15, 2022 |
| Title |
Single embryo RNA-seq of control and hydroxyurea treated embryos produced by nuclear transfer of cumulus cells. Single cell RNAseq of cumulus cells was performed using the same protocol. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Slow fork speed inducer, hydroxyurea, efficiently improved development of somatic cell nuclear transfered embryos to the blastocyst stage. Here, we performed single embryo RNA-seq approach to investigate whether nuclear transfer embryos exhibited a transcriptional programme similar to in vivo fertilised embryos.
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| Overall design |
RNA-seq of control and hydroxyurea treated nuclear transfered embryos at the 2-cell stage (28 hours post activation) was performed using SMART-seq2. RNA from single cumulus cells was performed using the same protocol. RNA was sequenced with NetSeq.
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| Contributor(s) |
Nakatani T, Yamagata K, Altamirano-Pacheco L, Tokoro M, Hatano Y, Torres-Padilla ME |
| Citation missing |
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| |
| Submission date |
Feb 08, 2021 |
| Last update date |
Mar 15, 2022 |
| Contact name |
Maria Elena Torres-Padilla |
| E-mail(s) |
torres-padilla@helmholtz-muenchen.de
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| Organization name |
Helmholtz Zentrum München
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| Department |
Institute of Epigenetics and Stem Cells
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| Street address |
Feodor-Lynen-Strasse 21
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA700563 |
| SRA |
SRP305294 |
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