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| Status |
Public on Feb 05, 2021 |
| Title |
miRNA profiling of putative biomarker candidates in Mycobacterium avium subsp. paratuberculosis-infected cattle |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease that is a chronic wasting disease in ruminants. Spread of MAP is mainly provoked by a long subclinical stage during which MAP is shed into the environment with feces; accordingly, detection of subclinical animals is very important to its control. Therefore, the current diagnostic methods are not suitable for detection of subclinical animals. In this study, we conducted to develop a diagnostic method for analysis of the expression of genes of prognostic potential biomarker candidates in cattle naturally infected with MAP. We conducted miRNA-Seq to evaluate potential biomarker candidates. Experimental animals were divided into four groups based on fecal MAP PCR, serum ELISA, and clinical sign.
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| Overall design |
miRNA-Seq were analyzed in four groups divided into fecal MAP PCR, serum ELISA, and clinical sign.
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| Contributor(s) |
Choi SW, Yoo HS |
| Citation(s) |
34735546 |
| |
| Submission date |
Feb 04, 2021 |
| Last update date |
Nov 10, 2021 |
| Contact name |
Sungwoon Choi |
| E-mail(s) |
chltjddns7@gmail.com
|
| Phone |
01020167484
|
| Organization name |
Seoul National University
|
| Department |
Veterinary Medicine
|
| Lab |
Infectious Disease
|
| Street address |
1. Gwanak-ro
|
| City |
Seoul |
| State/province |
Gwanak-gu |
| ZIP/Postal code |
ASI/KR/KS013 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA699634 |
| SRA |
SRP304803 |
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