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Series GSE165816 Query DataSets for GSE165816
Status Public on Nov 01, 2021
Title Single Cell Transcriptomic Landscape of Diabetic Foot Ulcers
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Diabetic foot ulcers (DFUs) are a devastating complication of diabetes. In order to identify systemic and local factors associated with DFU healing, we examined the cellular landscape of DFUs by single-cell RNA-seq analysis of foot and forearm skin specimens, as well as PBMC samples, from 10 non-diabetic subjects, and 17 diabetic patients, 11 with, and 6 without DFU. Our analysis shows enrichment of a unique inflammatory fibroblast population in DFU patients with healing wounds. The patients with healing DFUs also depicted enrichment of macrophages with M1 polarization, as opposed to more M2 macrophages in non-healing wounds. These findings were verified using Immunohistochemistry and Spatial Transcriptomics.
 
Overall design Single-cell RNA sequencing of foot and forearm skin cells, as well as peripheral blood mononuclear cells (PBMCs), from 10 samples from non-diabetic subjects, and 17 diabetic patients, 11 with, and 6 without DFU.

***Raw data not available due to patient privacy concerns***
 
Contributor(s) Bhasin M, Veves A
Citation(s) 35013299
BioProject PRJNA695306
Submission date Jan 29, 2021
Last update date Jan 12, 2022
Contact name Debasree Sarkar
E-mail(s) sreesarkar@gmail.com
Organization name Emory University
Department Pediatrics
Lab Lab 135
Street address 615 Michael Street
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (54)
GSM5050521 G1: Forearm skin of Healthy non-diabetic subject
GSM5050522 G1A: Foot skin of Diabetic subject without DFU
GSM5050523 G2: Foot skin of subject with healing DFU

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Supplementary file Size Download File type/resource
GSE165816_RAW.tar 391.2 Mb (http)(custom) TAR (of CSV)
Raw data not provided for this record
Processed data provided as supplementary file
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