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Series GSE165632 Query DataSets for GSE165632
Status Public on Mar 03, 2021
Title Non-coding RNAs in the transcriptional network that differentiates skeletal muscles of sedentary from long-term endurance- and resistance-trained elderly (microarray)
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary In a previous study, the whole transcriptome of the vastus lateralis muscle from sedentary elderly and from age-matched athletes with an exceptional record of high-intensity, life-long exercise training was compared, the two groups representing the two ends of a physical activity scale. Exercise training enabled the skeletal muscle to counteract age-related sarcopenia by inducing a wide range of adaptations, sustained by the expression of protein-coding genes involved in energy handling, proteostasis, cytoskeletal organization, inflammation control, and cellular senescence. Building on the previous study, we examined here the network of non-coding RNAs participating in the orchestration of gene expression and identified differentially expressed micro- and long-non-coding RNAs and some of their possible targets and roles. Unsupervised hierarchical clustering analyses of all non-coding RNAs were able to discriminate between sedentary and trained individuals, regardless of the exercise typology. Validated targets of differentially expressed miRNA were grouped by KEGG analysis, which pointed to functional areas involved in cell cycle, cytoskeletal control, longevity, and many signaling pathways, including AMPK and mTOR, which had been shown to be pivotal in the modulation of the effects of high-intensity, life-long exercise training. The analysis of differentially expressed long-non-coding RNAs identified transcriptional networks, involving lncRNAs, miRNAs and mRNAs, affecting processes in line with the beneficial role of exercise training.
Overall design all subjects were aged men (65 to 80 years); 5 were fully sedentary subjects; 4 were exceptionally trained subjects practicing mostly resistance exercise; 5 were exceptionally trained subjects practicing mostly endurance exercise
Contributor(s) De Sanctis P, Filardo G, Abruzzo PM, Astolfi A, Bolotta A, Indio V, Di Martino A, Hofer C, Kern H, Lofler S, Marcacci M, Marini M, Zampieri S, Zucchini C
Citation(s) 33546468
Submission date Jan 27, 2021
Last update date Mar 03, 2021
Contact name Valentina Indio
Organization name University of Bologna
Street address via Giuseppe Massarenti,11
City Bologna
State/province Bologna
ZIP/Postal code 40127
Country Italy
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (14)
GSM5047158 muscle of sedentary aged subject # 1
GSM5047159 muscle of sedentary aged subject # 2
GSM5047160 muscle of sedentary aged subject # 3
This SubSeries is part of SuperSeries:
GSE165633 Non-coding RNAs in the transcriptional network that differentiates skeletal muscles of sedentary from long-term endurance- and resistance-trained elderly
BioProject PRJNA695371

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Supplementary file Size Download File type/resource
GSE165632_RAW.tar 10.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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